biochemical and genotoxic effects of occupational exposure to vinyl chloride monoer

This study aimed to investigate the biochemical and genotoxic effects of the occupational exposure to vinyl chloride monomer [VCM]. The study included 84 workers occupationally-exposed to VCM, including 54 working in the Petrochemical Company and 30 working in El-Masria Company, in addition to 20 subjects as a control group. The biochemical assessment of the occupationally-exposed group involved a determination of glutathione [GSH] content in whole blood, erythrocyte glutathione peroxidase [GPx] and glutathione S-transferase [GST]. Twenty workers showed an elevation in these biochemical parameters. They were subjected to cytogenetic investigations including blood cultures for sister chromatid exchange [SCE] evaluation and micronucleus [MN] assay. A cytogenetic evaluation was performed to six of the control group using both SCE evaluation and MN assay. The statistical analysis of variance between groups [one-way ANOVA] revealed a significant decrease in GSH and GPx levels in the blood of workers when compared with the control group and a significant increase in the level of GST in workers when compared with the control group. The mean SCE/cell for the exposed workers was higher than that of the control, the difference was statistically nonsignificant. The mean MN/500 cytokinesis blocked binucleated cells was higher in the occupationally-exposed workers as compared the control group, the difference was statistically significant

effect of dimethylnitrosamine on the activities of renal puenolsulfotransferase and arylsulfatases A and B in schistosoma mansoni infected mice

The activities of renal phenolsulfotransferase and arylsulfatases A and B were estimated in 400 male Swiss albino mice classified into four groups: Normal controls, Schistosoma mansoni infected group, Dimethylnitrosamine [DMN] treated group and infected treated group. The activity levels of the studied enzymes were significantly increased in all groups when compared with the control group, also the statistical analyses showed a high significant increase of the three enzymes levels in the infected treated group; when compared separately with treated or infected groups. It was concluded, therefore, that schistosomal infection is implicated in the development of kidney cancer which may arise from the pattern of hepatic mixed-function oxidase induction characterized for schiatosomiasis and its temporal relationship with the procarcinogenic initiating events. Furthermore, the striking significant increase in the enzymatic activity levels of the acid hydrolases arylsulfatases due to the lesion of both cytotoxic effects of dimethylnitrosamine as well as pathological change of schistosomiasis which may play an active role in the initiation of the malignant process by detoxifying endogenous sulfated aromatic metabolites

The lipid profile of the sera of patients with Buerger's disease and atherosclerosis

Thirty patients with Buerger's disease aged between 27- 45 years, and 30 other patients with atherosclerosis aged between 49-70 years were studied for their lipid profile compared to two corresponding control groups of similar age and sex. Total lipids and triglycerides concentrations were significantly increased in the sera of all atherosclerosis patients and exhibited normal levels in the sera of Buerger's disease, while total phospholipids, phosphatidyl ethanolamine and lipid peroxides were increased in both Buerger's and atherosclerotic patients. Sphingomyelin increased in Buerger's disease and decreased in atherosclerosis, Lysolecithin did not change in Buerger's disease but decreased in atherosclerosis. On the contrary, phosphatidyl choline decreased in Buerger's disease and did not change in atherosclerosis. However total cholesterol did not change significantly in both diseased groups. The aforementioned findings could be used as diagnostic parameters for Buerger's disease and as a discrimination tool to differentiate between Buerger's disease and atherosclerosis

Serum immunoglobulins levels and B-glucuronidase activity in hepatosplenic bilharziasis

B-glucuronidase enzyme and total immunoglobulin in patients with hepatosplenic bilharziasis was the subject of the present study. A positive correlation between them was encountered

effect of trichomonas infection on immunoglobulins and b-glucornidase enzyme in long terms pill users

Studies on immunoglobulins IgA and IgG and B-glucuronidase activity in the vaginal fluids as well as B-glucuronidase activity in the sera of trichomoniasis and non-trichomoniasis long term oral contraceptive users as compared with their corresponding normal control group revealed statistically significant increase. This may be attributed to endogenous hormonal deficiency and infection